Genotoxic And Oxidative Damage Related To
PM2.5 Chemical Fraction
Author(s)
Sa. Bonetta, V. Gianotti, D. Scozia, Si. Bonetta, E. Carraro,
F. Gosetti, M. Oddone & M. C. Gennaro
Abstract
Many studies have pointed out a correlation between airborne PM quantitative
exposure and health effects. The aim of this research is the investigation of the
role of the PM2.5 chemical fraction in the DNA damage induction in human cells
(A549). Air samples (PM2.5) were collected in different sites (urban, industrial
and highway) using a high–volume sampler. Organic and water-soluble extracts
of PM2.5 were tested on A549 cells to evaluate genotoxic and oxidative damage
using the Comet assay without and with formamido-pyrimidine-glycosylase
(Fpg). Organic and water extracts were analysed for determination of PAHs by
GC-MS methods and metals by the ICP-MS technique respectively. The PM2.5
organic extract of all the samples caused a significant dose-dependent increase of
the A549 DNA damage. The genotoxic effect was related to IPA PM2.5 content
and the highest effect was observed for the motorway site sample (65.03
CL/10m3) while the oxidative damage was observed in PM2.5 water extract of the
industrial and motorway sites. The extent of the oxidative damage seems to be
related to the type and concentration of metals present in these samples. The
results of this study emphasize the importance of evaluating the PM chemical
composition for the biological effect determination. This concern highlights the
need for considering its qualitative composition in addition to its size and air
concentration for PM health effect evaluation and exposure management.
Keywords: PM, genotoxicity, oxidative damage, Comet assay, PAH, metals.
Keywords
PM, genotoxicity, oxidative damage, Comet assay, PAH, metals.
Related Book
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