Chestnut Bur Extracts As Antioxidants:
Optimization Of The Extraction Stage
Author(s)
G. Vázquez, A. Fernández-Agulló, M. S. Freire, G. Antorrena
& J. González-Álvarez
Abstract
In this work the exploitation and subsequent valorization of chestnut bur, a forest
waste product from chestnut processing in the food industry, is proposed. The
experimental design and response surface methodology were applied to optimize
the extraction of chestnut bur polyphenols with aqueous methanol. An
incomplete factorial design 33 was applied to study the influence of temperature
(25-50-75ºC), time (30-75-120 min) and methanol concentration (50-70-90% by
weight) on extraction yield, extract total phenols content (using the Folin-
Ciocalteu method), FRAP antioxidant activity, DPPH and ABTS radical
scavenging activity. Temperatures in the range 60-75ºC, an extraction time of 75
min and MeOH concentrations in the range 50-60% led not only to extracts with
high antioxidant activity (30.89-36.32 g GAE/100 g extract for the total phenol
content, 2220-2292 nmol AAE/mg extract for FRAP antioxidant activity; 3.22-
3.40 mmol TRE/g extract for the DPPH assay and 3.26-3.45 mmol TRE/g extract
for the ABTS assay), but also to high extraction yields (15.06-19.30%). Chestnut
bur extracts were analysed by RP-HPLC-ESI-TOF, which revealed the presence
of gallic acid esters of glucose, ellagic acid and small proportions of quercetin
and quercetin-3β-D-glucoside.
Keywords: chestnut bur, antioxidant activity, phenolic compounds, experimental
design, response surface methodology, RP-HPLC-ESI-TOF.
1 Introduction
The growing interest in the research on natural antioxidants has been promoted
by the need to find natural substitutes for synthetic antioxidants that have been
Keywords
chestnut bur, antioxidant activity, phenolic compounds, experimental
design, response surface methodology, RP-HPLC-ESI-TOF
Related Book
Other papers in this volume
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