Measurement Of Diffusion Coefficients Of Humic Substances In A Hydrogel
And In Water By Fluorescence Correlation Spectroscopy
Author(s)
J.R. Lead & K.J. Mlkinson
Abstract
Measurement of diffusion coefficients of humic
substances in a hydrogel and in water by
fluorescence correlation spectroscopy
1 7
J.R. Lead and K. J. Wilkinson
* Division of Environmental Health and Risk Management, University of
Birmingham, U.K..
^CABE, University of Geneva, Switzerland.
Abstract
Diffusion coefficients of three different humic substances (HS) were measured in
both water and in agarose hydrogels. The three HS were standard Suwannee River
humic and fulvic acid [1] and a peat humic acid [2]. Measurements were made by
fluorescence correlation spectroscopy (PCS). In general, values of diffusion
coefficients were in the range of 1.9-2.5 x 10"*° nf s"\ for all of the HS under all
conditions (except a single peat derived HS at low pH). The small degree of
polydispersity of the HS (observed both in the autocorrelation functions of the PCS
and by atomic force microscopy, AFM) did not preclude the use of a single
diffusion coefficient to ex
Keywords
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