Enzyme Assays For Estimating First-order Rate Constants Of
1,2-dichloroethane Biodegradation In Groundwater
Author(s)
R. Reiss & P. Guerra
Abstract
Enzyme assays for estimating first-order rate
constants of 1,2-dichloroethane biodegradation
in groundwater
I Department of Biology,
New Mexico Institute of Mining and Technologv
Socorro, New Mexico, USA.
2 AMCE Earth & Environmental,
Albuquerque, New Mexico, USA.
Abstract
Conventional methods for estimating the biodegradation rate of 1,2-
dichloroethane contamination in the subsurface consist of measuring respiration
byproducts or culturing bacteria from aquifer in a reactor. Compared with these
conventional methods, assays using protein extracted from groundwater samples
offered comprehensive and rapid results. Two assays were developed 1) the
specific activity and 2) the kinetic rate constants for the rate-limiting, hydrolytic
enzyme, haloalkane dehalogenase. The first step in each assay was to extract
total protein from the entire consortium of microbes in a fresh groundwater
sample. This was accomplished by centrifuging the groundwater sample,
resuspending and sonicating the pellet, and
Keywords
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