Development Of A Biomarker To Detect Bird
Fecal Waste In Environmental Waters
Author(s)
E.C. Chern & B.H. Olson
Abstract
Molecular microbial source tracking methods have been developed to identify
fecal waste sources entering environmental waters. However, most, such as
ribotyping and rep-PCR, rely upon library databases (Hoi et al. [1]; Sikorski et
al. [2]). These methods generally are only reliable in a specific geographic
region. Also, there is overlap amongst hosts, requiring manipulation of sample
isolates to eliminate this segment of the population. Recently, the library
independent biomarker method based on toxin genes associated with the
diarrheic E. coli, has been successful in identifying human, cow or pig waste
(Oshiro and Olson [3]; Khatib et al. [4], [5]). Using diarrheic E. coli as a model,
the temperature-sensitive hemagglutinin, tsh, gene associated with avian
pathogenic E. coli was tested as a b
Keywords
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