A Liquid Chromatography-tandem Mass
Spectrometry Method For The Analysis Of
Biomarkers Of Acrylamide Exposure
Author(s)
M. Ospina, H. Vesper, T. Meyers, A. Smith, G. Gray, L. Ingham
& G. Myers
Abstract
Acrylamide is a widely used industrial chemical. It has attracted attention since
being found to occur in a variety of fried and oven-baked foods. Acrylamide is a
known neurotoxin shown to be carcinogenic in animals. It is classified as a
potential human carcinogen. Acrylamide and one of its main metabolites,
glycidamide, form adducts with hemoglobin, which can be used as biomarkers of
acrylamide exposure. More data are need to ascertain the effects of acrylamide
on public health. Current methods have been designed to measure acrylamide in
exposed people with high acrylamide biomarker concentrations. However,
biomarker levels in the general population are significantly lower. Therefore, one
critical aspect for measuring acrylamide in the general population is appropriate
sensitivity of the instruments used. We performed systematic liquid
chromatography-mass spectrometry (LC/MS/MS) studies to determine the
optimal instrument conditions using commonly used techniques and newly
developed LC/MS/MS conditions. Results from different ionization techniques
and conditions show that the sensitivity can be increased sixfold using
atmospheric pressure chemical ionization instead of electrospray ionization.
Sensitivity was improved another tenfold by using a redesigned instrument.
Using 50 mg globin, the detection limits for these hemoglobin adducts are 7
pmol/g globin for glycidamide adducts and 3 pmol/g globin for acrylamide
adducts.
Keywords: biomarkers of exposure, hemoglobin adducts, mass spectrometry.
Keywords
biomarkers of exposure, hemoglobin adducts, mass spectrometry.
Related Book
Other papers in this volume
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