The Effect Of Implemented Pulsed Electric Field
(PEF) Treatment On The Dehydrogenase Activity
Of Activated Sludge
Author(s)
U. Koners, W. Schmidt, M. Löffler, V. Heinz & D. Knorr
Abstract
The implementation of sludge disintegration in wastewater treatment processes is
a possible strategy of sludge minimisation. Many sludge disintegration
technologies are being explored. A new additional method of disintegration is
the application of pulsed electric fields (PEF). In the PEF technique short high
voltage pulses are applied to generate an electric field in the sample, which
builds up an electrical potential difference between the inner and outer side of
the membrane. By exceeding a critical potential a perforation of the membrane is
evoked (Zimmermann et al. [1]). A benefit of PEF application on subsequent
aerobic and anaerobic treatment processes is shown (Koners et al. [2], Kopplow
et al [3]).
In this research the attention is turned to the release of cell content and change
in bio-activity of the sludge after PEF implementation. The most common tests
to determine the disintegrative effect is the release of dissolved organic carbon
(DOC) or the uptake of chemical oxygen demand (COD). As to determine
additionally sludge activity a dehydrogenase assay (DHA) with the
oxidoreduction dye resazurin was chosen. The DHA data are not dependent on
the metabolism of a nutritive additive like glucose. DHA can be a useful
monitoring parameter for sludge activity, though it correlates not always to
found respiration activity (Strotmann et al. [4]). According to Ewald et al. [5]
DHA with resazurin generally is a quick test for cell viability.
The disintegrative effect of PEF implementation was tested by DOC and
DHA and compared to each other. The test leads to the conclusion that for each
energy input the fraction of inactivated micro-organism keeps in the same range,
unaffected of the dry substance content. Consequently PEF implementation is
more energy efficient when the content of dry substances is high.
Keywords: disintegration, pulsed electric field, PEF, dehydrogenase assay,
DHA, resazurin.
Keywords
disintegration, pulsed electric field, PEF, dehydrogenase assay,DHA, resazurin.
Related Book
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