Mutation Of RNA Polymerase -subunit Confers
In Both Enhancement Of Environmental
Bioremediation And Antibiotic Production In A
Streptomyces Species
Author(s)
S. Rachid
Abstract
Research on environmental bioremediation has intensified in recent years
because of a continuous increase in environmental pollution accidents. Scientists
taking advantage of the microbial astonishing steps involved in metabolism and
breakdown of toxic macromolecules to clean up our environment from
pollutants. In this work some antibiotic producing Streptomyces inhabiting
contaminated soil with crude petroleum ”near Shiwashok oil field-Koya\“ were
isolated, and characterized based on the morphological properties and sequence
analysis of 16s rRNA coding region. The ability of the isolates was screened for
antibiotic production by in vitro antagonism against Gram positive and Gram
negative bacteria. Although the isolates were able to survive as free living
organisms in the petroleum contaminated soil, only one isolate was able to grow
and reduce the toxic hydrocarbons content in a synthetic media contains crude
petroleum as a sole source of carbon. Mutation in the rpoB gene encoding RNA
polymerase -subunit (1280 A-T, and 1319 G-A) that confers bacterial resistance
to rifampicin, resulted in significant increase of hydrocarbons degradation, and
apparent increase in the antibiotic production by the Streptomyces isolate.
Keywords: Streptomyces, bioremediation, rpoB mutation, antibiotic production.
Keywords
Streptomyces, bioremediation, rpoB mutation, antibiotic production.
Related Book
Other papers in this volume
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