Self Assembling Proteins As Matrix For The
Construction Of Optical Devices
Author(s)
U. Weinert, T. Günther, J. Raff & K. Pollmann
Abstract
Self-assembling biomolecules are widespread in nature and attractive for
technical purposes due to their size and highly ordered structures in nanometer
range. Surface-layer (S-layer) proteins are one of those self-assembling
molecules and their chemical and structural properties make them quite attractive
for nanotechnical purposes. They possess a high content of functional groups so
a sequential coupling of functional devices is possible and their ability to self
assemble in aqueous solutions or on surfaces, e.g. SiO2 wafers, qualifies them for
nanotechnical applications. In this work, first experiments were done in order to
construct a sensory device containing S-layer proteins as matrix to bind optical
elements and analytes for detection of specific substances. The S-layer proteins
were isolated from the Lysinibacillus sphaericus strain JG-A12 recovered from a
uranium mining waste pile in Germany. As optical elements fluorescent dyes or
quantum dots can be used. Three different fluorescent dyes which are able to
perform a Fluorescence resonance energy transfer (FRET) were used and
coupled to the S-layer proteins. As receptor molecule aptamers were chosen due
to their high specifity and stability towards many chemicals. Aptamers are short
oligonucleotides which are able to bind specific molecules via their three
dimensional structure. In this work, a model aptamer was used that is specific
towards human thrombin. The aim was to construct a sensor system which is
able to detect specific substances in very low concentration ranges in aqueous
solutions.
Keywords: S-layer proteins, fluorescent dyes, aptamers.
Keywords
S-layer proteins, fluorescent dyes, aptamers
Related Book
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