Acetanilide Herbicide Degradation Using
Indigenous Soil Microorganisms
Author(s)
S. Farooq, I. Hashmi, M. Arshad & I. A. Qazi
Abstract
A lab scale study was conducted to investigate the degradation of S-Metolachlor
(82.1 % W/W) using indigenous soil culture. S-Metolachlor is a pre-emergent
acetanilide herbicide for the control of annual grasses and broadleaf weeds.
Health advisory level (HAL) of S-Metolachlor by US EPA is 0.525 mg/L in
drinking water. The bacterial culture capable of degrading S-Metolachlor was
isolated from soil with the help of an enrichment technique. Isolated bacterial
cultures were identified as Pseudomonas sp. by using morphological and
biochemical characteristics. Molecular identification of Pseudomonas sp. with
the help of Polymerase Chain Reaction (PCR) was also conducted. Pure culture
of Pseudomonas aeruginosa (ATCC 27853) was used to determine its
degradation capacity. By using incubator an orbital shaker bacterial degradation
capacity at 10, 20 and 50μg/ml were determined. Gas chromatography and total
organic carbon (TOC) were used to determine the concentration of
S-Metolachlor at varied time intervals. TOC of S-Metolachlor was found to be
169.6 ppm. Isolated Pseudomonas sp. and standard culture of Pseudomonas
aeruginosa (ATCC 27853) showed higher degradation capacity of
S-Metolachlor.
Keywords: S-Metolachlor, biodegradation, Pseudomonas sp., acetanilide
herbicide, polymerase chain reaction, gas chromatography, enrichment
technique.
Keywords
S-Metolachlor, biodegradation, Pseudomonas sp., acetanilide
herbicide, polymerase chain reaction, gas chromatography, enrichment
technique
Related Book
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