Use Of The In Vitro Model For The Evaluation Of
Toxic Effects Of Metabolites Produced By Fungi
Author(s)
Z. Kováčiková, E. Piecková, E. Tátrai, Z. Pivovarová
& M. Mataušic-Pišl
Abstract
There are several reports about chronic intoxications, allergies or other
pulmonary health problems of dwellers (especially babies) and workers from
buildings contaminated with microfungi. Material from presented work was
collected during the study of indoor fungal colonization of mouldy dwellings and
public buildings in Slovakia. The isolated fungi were cultivated for 10 days in
liquid media and their products – both endo- and exometabolites were isolated.
The metabolites from four isolates of Stachybotrys chartarum, two of
Aspergillus versicolor and one of Penicillium chrysogenum were used. Their
effects were studied in vitro on rat alveolar epithelial type 2 cells, from the
toxicological point of view one of the most important lung cells type. Type 2
cells were cultured for 20 hours in DMEM and then they were exposed for
24 hours to various concentrations of metabolites. The toxicity was evaluated by
staining the cells for alkaline phosphatase which is a marker for this cell type.
The toxic effect of metabolites from all isolates of Stachybotrys chartarum was
dose dependent, there were differencies in the extent of cytotoxicity. From the
tested microorganisms Stachabotrys chartarum and Aspergillus versicolor
showed high toxic effects (Stachybotrys higher than Aspergillus), while
Penicillium chrysogenum only minor toxic effects in this test.
Keywords: alveolar epithelial type 2 cells, Stachybotrys chartarum, Aspergillus
versicolor, Penicillium chrysogenum, metabolites.
Keywords
alveolar epithelial type 2 cells, Stachybotrys chartarum, Aspergillus
versicolor, Penicillium chrysogenum, metabolites.
Related Book
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