Extraction Of Enzymes From Activated Sludge
Author(s)
D. Nabarlatz, J. Vondrysova, P. Jenicek, F. Stüber, J. Font,
A. Fortuny, A. Fabregat & C. Bengoa
Abstract
About 60–70% of the organic matter contained by domestic wastewater is
formed by lipids and proteins. The microorganisms that are able to degrade this
organic matter produce hydrolytic enzymes that cannot be produced by standard
cultivation techniques. The extraction methods have to include detergents or
ionic exchange resins to recover qualitatively and quantitatively the enzyme
activity. In the present study protease was extracted by using Triton X100 alone
or in combination with a cation exchange resin (CER), under stirring, mechanical
or ultrasound disintegration at low temperature. It was observed that the
temperature of extraction, the agitation velocity, the concentration of the
detergent or the cation exchange resin, and the presence/absence of a buffer
conditioned the efficiency of the extraction process and the recovery of the
enzyme activity. It was also demonstrated that the preservation of the sample
(cooled or frozen), and the time of withdrawal (immediately or after one day
aeration) changes the activity of the enzyme recovered. The results showed that
the best treatment for the recovery of protease is a combination of 60 g/g VSS of
CER, 0.5% Triton and 10 mM Tris buffer pH 8, under stirring at 500 rpm during
1 h at 18ºC. This process allowed to recover 6.48 protease units/g VSS.
Keywords: sludge, extraction of enzymes, protease, ultrasound disintegration,
mechanical disintegration, stirring, cation exchange resin, Triton X100.
Keywords
sludge, extraction of enzymes, protease, ultrasound disintegration,
mechanical disintegration, stirring, cation exchange resin, Triton X100.
Related Book
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