Potentiometric Biosensor For Acrylamide
Determination In Wastewater Using Wild Type
Amidase From Pseudomonas Aeruginosa
Author(s)
N. A. F. Silva, D. Gil, A. Karmali & M. Matos
Abstract
Acrylamide is a toxic amide with potentially hazardous effects on the
environment and human health. This paper reports the results regarding the
development of a potentiometric biosensor in order to determine the amount of
this amide in wastewater samples. The biosystem consisted of whole cells of
Pseudomonas aeruginosa containing intracellular amidase activity which
hydrolyses acrylamide producing ammonium ion and acrylic acid. The cells were
immobilized on the surface of several types of membranes such as
polyethersulfone, nitrocellulose and nylon, in the presence of glutaraldehyde as
bifunctional reagent, and then attached to the surface of an ammonium ion
selective electrode. Polyethersulfone was revealed to be the most adequate in
terms of biosensor response. The effect of glutaraldehyde concentration was also
studied and 5% (v/v) was chosen as the optimum concentration value. The
results obtained revealed excellent analytical characteristics of the biosensor
such as good linear response in the range of 0.5 to 100mM of acrylamide, a
detection limit of 4.48 x 10-5M, a response time of 55 s, a sensitivity of
58.9mV/mM. This system was also tested in real samples of complex matrix,
namely wastewater from an industrial plant where an average substrate recover
of 93.3% was obtained.
Keywords: acrylamide, potentiometric biosensor, wild-type amidase; whole
cells, Pseudomonas aeruginosa, wastewater.
Keywords
acrylamide, potentiometric biosensor, wild-type amidase; whole
cells, Pseudomonas aeruginosa, wastewater.
Related Book
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