In Vitro Toxicity Of Indoor Fungi From Dwellings
In Slovakia: Testing On The Isolated Lung Cells
Author(s)
Z. Kováčiková, E. Tátrai, E. Piecková, Z. Kolláriková,
V. Jančinová, J. Tulinská, M. Kuricová & A. Líšková
Abstract
The lung is the target organ of multiple aggressions due to environmental
noxious substances, indoor pollution, occupational hazards and personal risk
such as cigarette smoke. Metabolites produced by different fungus species can
also be present the inhaled air. Their effects are not frequently studied in the lung
or in the lung cells. Our study focused on the effects of metabolites (both exo-
and endometabolites) produced by Aspergillus ustus, Aspergillus versicolor,
Penicillium chrysogenum and Stachybotrys chartarum isolated from dwellings in
Slovakia. Their effects were studied in vitro on alveolar macrophages and
epithelial type II cells isolated from Wistar rats and Clara cells isolated from
mice. Alveolar macrophages represent a free living population in the alveolar
spaces and play an important role in maintaining clean and sterile alveoli; they
contribute also to the production of proinflammatory cytokines. Epithelial type II
cells play a critical role in preserving the functional integrity of the alveolar
surface and they also produce cytokines. The effects of metabolites were
evaluated by estimating their cytotoxicity, the activity of lysosomal enzyme acid
phosphatase in alveolar macrophages. Lectins were used for studying the
changes on cell surface. The production of cytokines (Monocyte Chemoatractant
Protein 1- MCP-1 and Tumor Necrosis Factor α – TNF-α) was also measured.
The effects of metabolites were dose dependent, the highest toxicity was evoked
by Stachybotrys chartarum metabolites.
Keywords: indoor fungi, lung cells, metabolites, in vitro.
Keywords
indoor fungi, lung cells, metabolites, in vitro.
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