The Influence Of Dissolved Oxygen On Growth
And Degradation Of Cypermethrin By
Pseudomonas Using A Biosimulator
Author(s)
S. Jilani
Abstract
Cypermethrin, a least water soluble pesticide, was selected because such
compounds are very difficult to remove from environmental systems by
conventional means. In this study, Pseudomonas strain (IES-Ps-1) was used to
assess its potential for Cypermethrin degradation. Continuous agitation of a
wastewater sample in biosimulator was found to be suitable for growth as well as
the metabolism of Cypermethrin by Pseudomonas. At optimum temperature (28-
30ºC) using 8-9 mg/L dissolved oxygen (DO), >85% degradation of
Cypermethrin was achieved after 48 hours when the initial added concentration
was 80 mg/L. In contrast, at 5-6 mg/L DO, only 38% degradation occurred.
When the concentration of DO further increased from 9 to 12mg/l, no
pronounced effect on the removal efficiency was observed. Results were
confirmed by COD and HPLC analysis. Moreover, during treatment, no
significant effect of pH change was observed and the pH remains between 7.3
and 8.8. The findings suggest that IES-Ps-1 strain could effectively be used for
the removal of pesticide waste.
Keywords: Cypermethrin, Pseudomonas, degradation, agitation, wastewater,
biosimulator, dissolved oxygen, pesticide waste.
1 Introduction
In Pakistan, insecticides, particularly Cypermethrin, are mainly used for cotton
crop protection and for forestry and public health management. The very low
water solubility of Cypermethrin and relatively high lipoaffinity indicate a strong
bioconcentration potential in aquatic organisms when available as solute [1,2].
Keywords
Cypermethrin, Pseudomonas, degradation, agitation, wastewater,
biosimulator, dissolved oxygen, pesticide waste.
Related Book
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