Occurrence Of Pseudomonas Aeruginosa In
Kuwait Environment
Author(s)
A. Akbar, D. AL-Otaibi, H. Drobiova, C. Obwekue & E. Al-Saleh
Abstract
Deliberate and accidental releases of opportunistic pathogens such as
Pseudomonas aeruginosa into the environment increase the likelihood of
spreading infectious diseases. Also, P. aeruginosa is responsible for nosocomial
infections and usually harbour several antibiotic resistance genes. In the present
study total of 76 strains of P. aeruginosa were characterized (54 strains were
isolated from soil samples collected from a crude oil contaminated site located
north of Kuwait and 22 strains were isolated from medical samples). Whereas
the phenotypic characterization such as the biochemical tests using VITEK 2
system (bioMérieux) and antibiotics susceptibility testing (AST) showed the high
similarity between the medical and the environmental strains, the molecular
analyses based on multilocus sequence typing (MLST) and restriction fragment
length polymorphism (RFLP) demonstrated the heterogeneity of isolated P.
aeruginosa indicating the presence of different clusters. Comparison of
fingerprints obtained, confirmed the negative correlation between the
environmental and the medical strains. In addition, results demonstrated that
environmental strains are better adapted to crude oil contaminated sites.
However, environmental strains may represent hidden reservoirs for pathogenic
traits that may disseminate to other bacteria in the environment.
Keywords: Pseudomonas aeruginosa; pathogenicity; MLST; RFLP; phenotype;
antibiotic.
1 Introduction
The versatile and ubiquitous bacterium Pseudomonas aeruginosa is one of the
most important opportunistic pathogens that can affect a wide variety of
Keywords
Pseudomonas aeruginosa; pathogenicity; MLST; RFLP; phenotype;
antibiotic.
Related Book
Other papers in this volume
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