Toxicity Of Volatile Organic Compounds (VOCs)
Mixtures Using Human Derived Cells
Author(s)
C. Khalil & J. Nasir
Abstract
Assessing the effects of contaminants is an issue of high priority for
governmental safety health and environmental agencies around the world. The
general conservative consensus is that chemicals in mixtures interact by
concentration addition. However, previous studies also report that concentration
addition of mixture components does not always reflect the overall toxicity of a
mixture. Volatile organic compounds (VOCs) such as Benzene, Toluene, Xylene
and Formaldehyde (BTXF) belong to the air pollutants found in urban and
indoor environments. They could trigger acute and chronic adverse health effects
like allergy, respiratory and cardiovascular diseases. The volatile nature of these
compounds poses additional problems in assessing individual volatile chemical
toxicity let alone mixtures of these chemicals. Our research aims at establishing
the true toxic effects of VOC exposure in vitro using a static direct exposure
glass-chamber method. This was achieved by assessing and comparing
individual and interactive effects of VOCs in exposed human epithelial lung
(A549) and liver cells (HepG2) using the MTS cytotoxicity assay to assess cell
viability upon VOC insult. The study results clearly indicated the limitation of
the concentration addition method used in assessing volatile mixtures
cytotoxicity and the need to develop new techniques for rapid and accurate
mixture toxicity determination. The study may have implications for regulatory
risk assessment of environmental volatile organic chemicals.
Keywords: static method, MTS, cytotoxicity, lung cells, liver cells, VOCs.
1 Introduction
Human environmental chemical exposures are characterised by exposures to
direct multiple chemical combinations or sequential exposure to individual or
Keywords
static method, MTS, cytotoxicity, lung cells, liver cells, VOCs
Related Book
Other papers in this volume
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