Correlation Between Cluster Analyses Of
Salmonella Strains Isolated From Diarrhetic
Patients In Kuwait And Biofilm Formation
Author(s)
A. Al-Mousawi, A. Eissa, F. Abu-Zant, H. Drobiova,
I. Al-Saif
& E. Al-Saleh
Abstract
Salmonella is a highly diverse group of strains that belong to the
Enterobacteriaceae and can cause many infections, such as diarrhea, pyrexia and
septicemia, in humans and animals. One important virulence factor is the ability
to form biofilm. In the present study, the potential to form biofilms by
Salmonella strains isolated from diarrhetic patients was investigated and
correlated with the strain type. Isolated bacteria were identified by sequencing of
16S rDNA. The potential of Salmonella to form biofilms was determined using
bioluminescence microbial cell viability assay. In addition, the metabolic
fingerprints of Salmonella were determined using the Biolog system, following
the manufacturer’s instructions. Cluster analysis based on catabolic activity and
16S rDNA of isolated strains showed the tendency of most Schwarzengrund
(66.7% - 70%) and E5 strains (85%) to cluster individually, which implied the
high distinctive genetic background of Schwarzengrund and E5 strains. On the
other hand, Heidelberg and Paratyphi strains were clustered among other stains,
which reflected the genetic resemblance of these strains to other Salmonella
strains. The biofilm studies showed the high potential of the majority of E6
(60%) and Heidelberg (66.7%) strains to form biofilms, while low potential to
form biofilms was displayed by 78% of Schwarzengrund strains.
Keywords: Salmonella, biofilm, 16S rDNA sequencing, metabolic fingerprint.
Keywords
Salmonella, biofilm, 16S rDNA sequencing, metabolic fingerprint
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