Set-up Of A Dynamic In Vitro Exposure Device For
The Study Of Indoor Air Pollutant Effects On
Human Derived Cells
Author(s)
F. Pariselli, M. G. Sacco & D. Rembges
Abstract
Exposure to air pollutants such as volatile organic chemicals (VOCs) is
recognized as a potential cause of allergies, asthma, mucous irritation, headaches
and tiredness and may substantially contribute to the increase of cancer incidence
in the population. Their presence is not limited to urban and workplace
environment but in homes, offices, schools, and hospitals at concentrations often
much higher than outdoors. As the number of chemical products created is still
increasing, the study of the toxic effects of VOC mixtures requires the
development of an effective and reproducible technique for in vitro exposure of
cell culture to air pollutants as an alternative tool to in vivo tests. The aim of this
work was to develop an in vitro exposure set-up for the assessment of the
toxicity of single volatile chemicals and their mixtures on representative VOCs
target tissues: skin and lung. We used a dynamic exposure module, named
CULTEX ® , where human derived cell lines from lung epithelium (A549) and
keratinocytes (HaCaT) were exposed in an air/liquid interface to a low flow of
air pollutants mixture as a close simulation of in vivo exposure. Exposure to
toluene or benzene concentrations showed reproducible and dose-related direct
toxic effects on both cell lines. Moreover, benzene or toluene induced an
inflammatory response. The results obtained so far show the sensitivity and
specificity of the overall CULTEX ® exposure module that could allow one to
conduct further investigations on the effects of mixture of air pollutants on these
representative target tissues.
Keywords: dynamic exposure device, inflammatory response, in vitro
cytotoxicity, volatile organic compounds.
Keywords
dynamic exposure device, inflammatory response, in vitro
cytotoxicity, volatile organic compounds.
Related Book
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