Isolation And Characterization Of Endophytic
Bacteria Of Coffee Plants And Their Potential In
Caffeine Degradation
Author(s)
F. V. Nunes & I. S. de Melo
Abstract
The plant kingdom is colonized by a diverse array of endophytic bacteria, which
form a non-pathogenic relationship with their hosts. Endophytic bacteria may
confer benefits to the plant, and the benefits may be reciprocal, resulting in an
enhanced symbiotic system for specific plant characteristics. The objective of
this study was to isolate endophytic bacteria from coffee (Coffea arabica and C.
robusta) and evaluate their potential in degrading caffeine. The isolates were
identified by partial 16S rDNA sequence analysis and by FAME and the analysis
of biodegradation of caffeine was carried out by HPLC. As a result, 252 bacterial
strains were isolated, with most of them belonging to the species Bacillus
lentimorbus, B. megaterium, B. subtilis, B. cereus, Pseudomonas putida, P.
chlororaphis, Pantoea ananatis, P. agglomerans, Stenotrophomonas
malthophilia, Kluyvera cryocrescens, Kocuria kristinae etc. Approximately 20%
of the bacterial strains showed the ability to grow in the presence of high
concentrations (5.000 mg.L -1 ) of caffeine and two Pseudomonas putida strains
completely degraded the alkaloid, showing the potential of endophytic bacteria
in decaffeination processes.
Keywords: endophytic microorganisms, caffeine, coffee, biodegradation.
1 Introduction
Endophytes are microorganisms that live within the tissues of healthy plants
where they cause symptomless infections. Their significance to the host is still
Keywords
endophytic microorganisms, caffeine, coffee, biodegradation.
Related Book
Other papers in this volume
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