Mechanical Characterization Of
Deep Vein Thrombosis In A Murine Model
Using Nanoindentation
Author(s)
K. C. McGilvray, R. Sarkar & C. M. Puttlitz
Abstract
Seventy percent or more of the blood volume is contained by the venous return,
and this system of vessels represents a highly distensible, low-pressure
configuration. Disease states associated with the venous system have a high
impact on society; complications from acute and chronic deep vein thrombosis
(DVT), the precursor to post-phlebitic veins (PPV), contribute to more deaths
each year than AIDS and breast cancer combined, affecting between 1 and 2% of
hospitalized patients in the United States. To our knowledge, there are no
published studies with regard to the biomechanical dispensability of either
clinical or experimental PPV. To examine the effects of DVT induction on
vessel wall biomechanics, a distinct DVT-induced murine model was evaluated.
The DVT model was generated using a well-established model via partial
ligation of the murine vena cava (MVC). The anisotropic biomechanical
properties of each variant were determined along the primary loading axes:
circumferential (perpendicular to the surface created by transecting the vein wall
longitudinally), longitudinal (perpendicular to the beginning/end surfaces of the
isolated intact vessel segment), and luminal (perpendicular to inner surface of
vessel) of healthy and diseased vascular tissue via nanoindentation. Three
indentation parameters were determined to describe the inherent tissue
mechanics: unloading stiffness and two reduced elastic moduli (Oliver-Pharr and
JKR formulations). Nanoindentation testing indicated that MVC induced with
DVT demonstrated mean deviations (as compared to the normal, baseline tissue)
in elastic moduli for the longitudinal and luminal directions. The data also
indicate that the unloading stiffness in the longitudinal direction has the largest
average magnitude among the three physiologically-relevant planes.
Keywords: nanoindentation, deep vein thrombosis, elastic modulus.
Keywords
nanoindentation, deep vein thrombosis, elastic modulus.
Related Book
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