Developing A Molecular Method For
Screening The Resistance To A Pathogen
Of Tomato Plants To Contribute To Limiting
The Use Of Toxic Chemicals In Soil
Author(s)
M. Aragona, A. Infantino & M. Papacchini
Abstract
Many soil-borne fungal pathogens cause severe diseases of many important
crops. Their control is mainly based on the application of toxic chemicals to the
soil, thus representing a serious problem for the environment. Pyrenochaeta
lycopersici, the cause of the corky root disease of plants both in the field and in
the greenhouse, is a well-studied pathogen of tomato plants. The most effective
way to resist the disease has been the use of methyl bromide, which is actually
banned, while other chemicals are less effective and still toxic for the
environment and the users. The identification and utilization of tomato plant
cultivars resistant to the disease represents a valid alternative to the use of
chemicals. With this objective, the research aimed to develop a molecular
method for the early screening of plants resistant to corky root to be used in
breeding programs. The method includes the artificial inoculation of the roots of
young tomato plants with a virulent P. lycopersici isolate expressing the β-
glucuronidase gene, obtained via genetic transformation of a wild fungal isolate.
The histochemical assay of infected roots showed that in resistant cultivars the
growth of the fungus is limited from the first stage of the infection. By this
method it should be possible to discriminate among susceptible and resistant
plants based on the quantification of β-glucuronidase differentially produced in
the infected roots of the tomato plant, in 15 days instead of the 40 days needed
with the conventional screening test used until now. The high accuracy of this
method will improve the screening procedures for the production of resistant
plants, allowing a useful reduction in the use of toxic chemicals in the cultivated
soils.
Keywords: tomato, disease resistance, infested soil, β-glucuronidase.
Keywords
tomato, disease resistance, infested soil, β-glucuronidase.
Related Book
Other papers in this volume
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